Reduction in Hg phytoavailability in soil using Hg‐volatilizing bacteria and biochar and the response of the native bacterial community

Summary Biological approaches are considered promising and eco‐friendly strategies to remediate Hg contamination in soil. This study investigated the potential of two ‘green’ additives, Hg‐volatilizing bacteria (Pseudomonas sp. DC‐B1 and Bacillus sp. DC‐B2) and sawdust biochar, and their combination to reduce Hg(II) phytoavailability in soil and the effect of the additives on the soil bacterial community. The results showed that the Hg(II) contents in soils and lettuce shoots and roots were all reduced with these additives, achieving more declines of 12.3–27.4%, 24.8–57.8% and 2.0–48.6%, respectively, within 56 days of incubation compared to the control with no additive. The combination of DC‐B2 and 4% biochar performed best in reducing Hg(II) contents in lettuce shoots, achieving a decrease of 57.8% compared with the control. Pyrosequencing analysis showed that the overall bacterial community compositions in the soil samples were similar under different treatments, despite the fact that the relative abundance of dominant genera altered with the additives, suggesting a relatively weak impact of the additives on the soil microbial ecosystem. The low relative abundances of Pseudomonas and Bacillus, close to the background levels, at the end of the experiment indicated a small biological disturbance of the local microbial niche by the exogenous bacteria.


Introduction
Soil pollution by heavy metals is a serious environmental problem worldwide . Elevated levels of heavy metals in soil pose a severe threat to ecosystem sustainability and human health through the food chain Wang et al., 2018). As one of most key global pollutants, mercury (Hg) attracts great concern due to its high mobility and toxicity, increased concentration and long retention in environments (Driscoll et al., 2013). Hg has been placed in the third position on the 'priority list of hazardous substances' by the Agency for Toxic Substances and Disease Registry (ATSDR, 2014). In addition to the reduction in Hg discharge from anthropogenic activities, development of effective techniques to remediate Hg-contaminated soil is urgently required for the protection of human and ecosystem health (Wang et al., 2012).
Conventional physicochemical approaches such as precipitation and elution with chemical reagents, thermal treatment, solidification and stabilization can effectively remove Hg from soil or transform it to other less toxic or immobile forms (Wang et al., 2012;Xu et al., 2015). Unfortunately, application of these technologies is usually restricted due to their high cost, secondary pollution and harmfulness to soil ecosystems (Mahbub et al., 2017a).
In recent years, the alternative use of eco-friendly bioagents to remediate Hg-polluted environments in situ, especially environments with large areas and low Hg concentrations, has attracted increasing interest. Of these bioagents, Hg-resistant microorganisms and biochar are two emerging and promising representatives (Park et al., 2011;Giovanella et al., 2017;Mahbub et al., 2017a;Chang et al., 2019). Specifically, certain microbes can resist Hg pollutants through transforming Hg into volatilizable Hg 0 with much less toxicity and solubility, which subsequently escapes from Hg-polluted water or soil employing the 'mer' operon (Barkay, 1987;Yu et al., 2014;Giovanella et al., 2016) or immobilizing Hg by various functional groups (Francois et al., 2012). To date, many Hg-resistant bacteria have been isolated; these organisms can remove Hg from solution via volatilization and/or biosorption (Giovanella et al., 2016), and a few have recently been used to enhance Hg removal from Hg-polluted soils (Mahbub et al., 2017b;McCarthy et al., 2017). Compared to absolute Hg concentration, reducing Hg phytoavailability in soil is more crucial because available Hg can be easily assimilated by plants and may enter the food chain. It is expected that Hg-volatilizing bacteria can reduce both the Hg content and phytoavailability in soils, but their potential for reducing Hg phytoavailability in soils has not been reported.
Biochar is a carbonaceous material produced by the pyrolysis of carbon-rich biomass under oxygen-limited conditions and can remove heavy metals from aqueous solution efficiently through adsorption (Inyang et al., 2016). A few recent studies reported that Hg bioavailability in sediment and soil could be decreased by biochar amendment, which is primarily attributed to ion exchange and the complexation of Hg by abundant functional groups such as hydroxy and carbonyl groups on biochar Wang et al., 2018). For soil, other agronomic and environmental advantages such as amelioration of fertility and structure, nutrient fixation, recycling of agricultural waste and carbon sequestration can also be supplied by biochar amendment (Laghari et al., 2016;Wang et al., 2018). The coapplication of biochar and metal-resistant bacteria for remediation of Cr-/Cd-contaminated soils has been investigated recently (Arshad et al., 2017;Li et al., 2017, and our previous work preliminarily showed the feasibility of a Hg-volatilizing bacterial strain and biochar to enhance Hg elimination from soils (Chen et al., 2018). However, little research has focused on the reduction of Hg phytoavailability in soil by biochar and microbes, which is more significant in the remediation of Hg-polluted soil, and further exploration is needed.
Microbial community structure is critical for the maintenance of the health and stability of soil ecosystems because of the key role of various microorganisms in matter cycling and ecosystem service supply in soil (Wu et al., 2016;Fischer et al., 2018). Additionally, the microbial community can serve as a bioindicator of environmental variation (Castro et al., 2010;Zhang et al., 2018). Hg pollution and the introduction of exogenous biomaterials for remediation such as biochar and microorganisms may influence and even disturb the soil microecosystem by changing soil properties and breaking the original microbial niche (Mahbub et al., 2017c;Zhang et al., 2018). Thus, the function and stability of the soil ecosystem may be adversely impacted, leading to some ecological risk. Unfortunately, relevant information is currently lacking. Therefore, the effect of Hgvolatilizing bacteria and biochar addition on the microbial community in Hg-polluted soil must be evaluated.
In this study, two strains of isolated Hg-volatilizing bacteria and sawdust biochar were added to Hg-polluted soil for remediation through pot experiments, and the response of the soil bacterial community to these additives was investigated. The main objectives were (i) to investigate the effectiveness of a combined Hg-volatilizing bacterial and biochar treatment for reducing Hg(II) phytoavailability in Hg-polluted soil and (ii) to evaluate the effect of these additives on the soil bacterial community.

Effect of Hg-volatilizing bacteria and biochar additions on Hg contents in soils and lettuces
The Hg(II) contents in soils and lettuces receiving different treatments are shown in Fig. 1.
The Hg(II) content in the control soil without additives decreased by 39.1%, which might have resulted from abiotic and biotic processes (Schl€ uter, 2000). Reductants such as Fe 2+ and dissolved organic compounds may be mainly responsible for the abiotic reduction of Hg(II) (Hori et al., 2015), and indigenous microbiota can contribute to Hg elimination (Barkay, 1987). Hg in soil could also be assimilated by the lettuces, but this could be ignored (< 1.0%) because of the small amount of lettuce biomass (Table 1). High Hg losses, accounting for 20-62% of the total initial Hg in soils freshly contaminated with soluble Hg compounds through volatilization during a growing season, were reported in a previous study (Han et al., 2006). The amendments of biochar and/or Hg-volatilizing bacteria DC-B1 and DC-B2 promoted Hg removal from the soils by 12.3-27.4% (P < 0.05), with higher efficiency in the microcosms amended with 4% biochar (66.5%) and a combination of 4% biochar and DC-B2 (63.8%), indicating the ability of these bioagents to reduce Hg content in Hg-polluted soil in situ. Biochar can act as a reductant by providing electrons and redoxreactive compounds (e.g. hydroquinone) (Oh et al., 2012;Wang et al., 2018) and offers habitats for microbes and their interactions, which might greatly facilitate Hg reduction. Chen et al. (2016) reported that biochar amendment stimulated bioreduction of Fe(III) and As(V) in sediment. Moreover, soil porosity and aeration can be enhanced by biochar amendment, which facilitates Hg volatilization from soil to air (Laghari et al., 2016). Additionally, the Hg volatilization ability of DC-B1 and DC-B2 performed to reduce the Hg content in Hg- polluted soil (55.1% and 53.3%, respectively), although the combination of biochar and Hg-volatilizing bacteria did not achieve higher Hg removal efficiency as expected, suggesting their potential for augmenting Hg decontamination in soil, which is consistent with recent studies (Mahbub et al., 2017b;McCarthy et al., 2017). In addition to the reduction in Hg(II) content in soils, Hg (II) uptake by the lettuce shoots was also significantly decreased (24.8-57.8% relative to the control, P < 0.05) in the biochar and/or Hg-volatilizing bacteria soil treatments. The lowest Hg(II) uptake by shoots (7.0 AE 0.6 mg kg dw À1 ) was observed in the treatment with coapplication of 4% biochar and DC-B2, although there was no statistically significant difference between this treatment and the treatments with 1% biochar (9.1 AE 3.8 mg kg dw À1 ) and 4% biochar (9.8 AE 1.2 mg kg dw À1 ). Furthermore, Hg(II) accumulation in lettuce roots also decreased under various treatments (2.0-48.6% relative to the control), although the difference between certain treatments was not significant (control vs. DC-B1, DC-B2 and 1% biochar addition, P > 0.05). For comparison, 4% biochar and the combination of 4% biochar and DC-B2 were better for the remediation of Hg-polluted soil. In addition to facilitating Hg loss from soils, biochar can bind Hg in a more stable form than unbound Hg by providing abundant functional groups (Inyang et al., 2016;Wang et al., 2018), lowering the transport and bioavailability of Hg in the soil system. Soil pH values increased from 4.7 to 5.0 and 5.8, respectively, with 1% and 4% biochar addition because of the alkaline nature of the biochar (pH = 9.96), which was conductive to the reduction in Hg bioavailability (Park et al., 2011;Arshad et al., 2017). DC-B1 and DC-B2 transformed Hg(II)  to Hg 0 and might bind a small portion of Hg(II) especially DC-B1, which is not assimilated by plants (Fern andez-Mart ınez et al., 2015), and consequently, Hg phytoavailability was reduced in soils. Nevertheless, soil is a highly complex ecosystem and the performance of these additives in Hg decontamination in soil can be influenced by many factors. In this study, biochar was more efficient in the remediation of Hg contamination in soil than the Hg-volatilizing bacteria, and certain treatment such as the combination of 4% biochar and DC-B1 did not perform well as expected, probably because of the relatively low biomass of bacterial inocula and low Hg volatilization ability and activity of DC-B1. Further studies on factors influencing the Hg remediation efficiency and corresponding optimization are required. Biochar amendment or bacterial inoculation has reduced plant uptake of heavy metals such as Cd, Cu and Cr (Arshad et al., 2017;Li et al., 2017Liu et al., 2018), but to the best of our knowledge, this report is the first concerning the feasibility of remediating Hg-contaminated soil from the view of Hg volatilization and reduction in Hg bioavailability.

Effect of biochar and bacterial biomass additions on lettuce growth
The biomass, shoot and root lengths of the lettuce decreased under the stress of Hg and increased in the majority of the microcosms with the additives, although the differences were almost not significant (P > 0.05 except a few treatments, Table 1). Taking the total Hg mass accumulated in lettuce with increased biomass into consideration, the additives also largely reduced Hg uptake by the lettuces. Moreover, protein content in the lettuce shoot from the treatments with biochar (8.46-11.43 lg g À1 ) was higher than that in the control (8.11 lg g À1 ), with significant increase (P < 0.05) in those amended with only biochar, although slight decreases were observed in the bacteria-added treatments (7.87-7.94 lg g À1 ). Chlorophyll content in the shoot from the microcosms with Hg-volatilizing bacteria and 1% biochar (0.12-0.14 mg g À1 ) also increased compared with that in the control (0.11 mg g À1 ), while it decreased slightly in other treatments. The lettuce growth parameters were not positively correlated with the Hg remediation efficiency, and lower values were even observed in certain remediated treatments than those in the control, partly as a consequence of the complex influence of the additives on soil properties and growing lettuce. However, the increase of lettuce growth parameters in some treatments indicated that the stress of Hg pollution in soil might have been mitigated by the additives. Biochar can improve soil properties to encourage plant growth, and bacteria belonging to the genera Pseudomonas and Bacillus may be phosphate-solubilizing and plant growth-promoting agents (Canbolat et al., 2006;Chen et al., 2006). Enhanced plant growth by biochar amendment and/or functional microorganism inoculation in heavy metal-polluted soil was also recorded in previous reports (e.g. Siripornadulsil and Siripornadulsil, 2013;Rees et al., 2016).

Effect of biochar and DC-B1/DC-B2 amendments on the soil bacterial community
A total of 308177 valid bacterial sequences were obtained from the soil samples by high-throughput sequencing of the 16S rRNA gene and quality control. The shapes of the rarefaction curves (Fig. S1) indicate that bacterial richness was completely sampled. The alpha diversity of the soil bacterial community is shown in Table 2. The bacterial richness was obviously lower in Hg-polluted soil than in clean soil, suggesting that the number of bacterial populations declined as a result of the Hg stress. The addition of 4% biochar best enhanced bacterial richness, followed by the addition of DC-B2 and 4% biochar, while 1% biochar amendment could hardly improve bacterial richness, which was consistent with the efficiency of Hg removal from soil by these treatments. The bacterial richness in DC-B1-added soils was lower than that in the control, probably because of the antibacterial effect of DC-B1. Similar bacterial diversity in all soil samples was obtained, indicating that Hg(II) and these additives had little influence  Rutigliano et al. (2014) and Papadopoulou et al. (2018) reported that biochar addition and bioaugmentation with an isolated thiabendazole-degrading bacterial consortium showed no effect on the soil bacterial diversity respectively. All of the gene sequences were classified from the phylum to the genus level, and the results are shown in Fig. 2. The overall compositions of the bacterial community in the soils under different treatments at the end of the experiment were similar, although the distribution of each phylum varied. Proteobacteria occupied the majority (59.2-60.2%) of the total bacterial populations and remained stable under different treatments, suggesting a lack of sensitivity of this phylum to Hg pollution and additives. A previous study also showed that Proteobacteria dominated in soil and was less affected by Hg pollution than other phyla (Mahbub et al., 2017c). The majority of the spiked Hg was bound with soil particles, and indigenous microbes possessing or developing mer operons could reduce part of the available Hg to gaseous Hg 0 (Barkay, 1987), resulting in a limited influence of Hg on soil bacterial community compositions. Mahbub et al. (2017c) observed that bacterial diversity decreased only at a Hg content of more than 50 mgÁkg À1 in soils. In addition, Bacteroidetes (10.8-15.4%), Actinobacteria (9.7-13.8%) and Gemmatimonadetes (4.4-5.3%) were dominant in the soil samples. The phylum Actinobacteria is often associated with the degradation of recalcitrant organic substances, and the increased relative abundance of Actinobacteria in the biochar-amended soil treatments might be derived from their ability to degrade recalcitrant carbon compounds (Lehmann et al., 2011). The relative abundances of the copiotrophic phyla Bacteroidetes and Gemmatimonadetes also increased slightly, and these findings were consistent with previous reports Sheng and Zhu, 2018). The relative abundance of Acidobacteria decreased slightly after biochar addition, probably because the alkaline environment created by biochar amendment is not beneficial for the growth of Acidobacteria which are usually acidophilic (Mao et al., 2012). Overall, the amendments of these additives did not alter the dominance of the main phyla in the short experimental time, indicating the elasticity of the bacterial community against the disturbance of exogenous introduction of biomaterials in soils.
The effect of biochar amendment on soil microbial community structure remains controversial. It was reported that wood biochar addition had little or no influence on soil microbial community compositions (Castaldi et al., 2011;Liu et al., 2017), while in other studies, it was proposed that biochar addition significantly altered microbial community structure and increased microbial activities Liao et al., 2016). This difference may be related to the varied soil and biochar properties and experimental conditions, including  (Lehmann et al., 2011;Rutigliano et al., 2014;Liu et al., 2017). In this study with a relatively short operation time, the dominant genera in biochar-added soils were similar to those in other soils, although their relative abundances shifted and the samples with biochar were clustered together as a group detected by the hierarchically clustered heat-map analysis (Fig. 3). Specifically, the growth of Asticcacaulis, Noviherbaspirillum, Luteimonas, Pseudarthrobacter and Shinella was stimulated by biochar amendment (Fig. 3). Among these bacteria, Pseudarthrobacter , Shinella (Bai et al., 2009) and Luteimonas (Liu et al., 2014) may be related to the degradation of recalcitrant aromatic compounds released from the added biochar (Sheng and Zhu, 2018). However, the effect of biochar on microbial populations is relatively limited because microorganisms have difficulty in using most OM from biochar in the short incubation period (Lehmann et al., 2011;Wu et al., 2016). A similar limited effect of biochar amendment on bacterial community structure in soil remediation has been reported previously .
The effect of the invasion of foreign microorganisms on the local microbiota has received increasing attention in recent years Papadopoulou et al., 2018). With the soil treatments introducing Pseudomonas sp. DC-B1 and Bacillus sp. DC-B2, the relative abundances of Bacillus and Pseudomonas were near the low levels (< 0.2%) found in the control (Table S1), indicating that the two added strains vanished at the end of this experiment, which probably indicated the ecological security of the added bacteria with weak influence on the soil microecosystem when applied in soils. A previous study also showed that the number of Pseudomonas veronii cells applied directly to Hg-contaminated soil declined rapidly in the first 3 weeks (McCarthy et al., 2017). Similarly, Lang et al. (2018) reported that the phylum Actinobacteria accounted for the majority of soil bacterial populations after inoculation with the strain AH-B (belonging to this phylum) for quinclorac degradation, while its relative abundance subsequently decreased to the low level in the control after 56 days of incubation. Homoeostatic mechanisms work to restore the disturbed microbial community to its previous state (Farinati et al.,  . However, further studies should be conducted to maintain the growth and activity of the added bacterial inocula in soils, such as cell immobilization by gel entrapment before addition , to maintain/ enhance the Hg remediation efficacy and reduce operation cost.

Conclusion
This work demonstrated the high potential of two biomaterials-Hg-volatilizing bacteria DC-B1/DC-B2 and biochar-as well as their combinations as additives for the in situ remediation of Hg-polluted soils by reducing the content and phytoavailability of Hg(II). Moreover, bioaugmentation with these exogenous bioagents displayed a marginal effect on native soil bacterial community structure and diversity, suggesting a weak impact of the additives on the soil microecosystem.

Experimental procedures Materials
Hg-volatilizing bacteria. Two Hg(II)-volatilizing bacterial strains, Pseudomonas sp. DC-B1 (GenBank No. MG754008, Chen et al., 2018) and Bacillus sp. DC-B2 (GenBank No. MG687381, unpublished data), isolated from a heavy metal-contaminated site previously, were used in this study. These strains displayed a strong ability to remove Hg(II) from culture solution predominantly by volatilization (Hg biosorption by DC-B1 and DC-B2 contributed < 15% and 2% for Hg removal, respectively), and the Hg volatilization ability of DC-B2 was greater than that of DC-B1. The preliminary study showed that these bacteria had the potential to enhance Hg(II) removal from Hg-polluted soils.
Biochar. Biochar was made by slow pyrolysis of pine sawdust, a by-product collected from a local woodprocessing factory, and its preparation procedures and basic properties were presented in a previous report (Chen et al., 2018). Two application rates of biochar (1% and 4% wÁw À1 of soil) were used.

Pot experiments for soil bioremediation
Soil sample. The soil sample used for the experiment was collected at depths of 0-5 cm from the Yunnan University campus located in Kunming, China (24°49 0 12″ N, 102°51 0 4.7″E). After air-drying and sieving through a 2-mm sieve, HgCl 2 solution was dosed into the soils to achieve a Hg content of 40 mg kg À1 (dry weight, DW) on the basis of the Hg concentration range in Hgcontaminated sites recorded in the literature (Qiu et al., 2005;Wang et al., 2012;Mahbub et al., 2017b). After fully homogenizing, the Hg-spiked soils were stored at room temperature of 20°C for 45 days with the water holding capacity (WHC) maintained at 40-60% for stabilization. Hg(II) content in the soil after ageing was measured to be 29.3 AE 0.13 mgÁkg À1 , suggesting that a fraction of the Hg had been volatilized. Basic physicochemical properties of the soil sample were determined based on the methods described previously (Yang et al., 2008;Chen et al., 2018) and listed as follows: pH 4.71 AE 0.08, organic matter content 0.23 AE 0.03%, available phosphate 1.21 AE 0.06 mgÁkg À1 , potassium 123.34 AE 6.81 mgÁkg À1 , alkaline nitrogen 83.7 AE 7.12 mgÁkg À1 and sandy loam texture.
Experimental setup. The bioremediation experiment was performed in a greenhouse at Yunnan University from October to December 2017. The Hg-containing soils (200 g) were put in polypropylene pots (length 9 width 9 height: 6 9 7.5 9 8.3 cm), and DC-B1 and DC-B2 inocula developed in LB medium overnight to early log phase were added to reduce Hg content and phytoavailability in soil at a dosage of 10 7 cfu g À1 soil (Arshad et al., 2017). In addition, two dosages of biochar, that is, 1% and 4% (w/w), were fully incorporated into the soils. Sterile distilled water was supplied regularly to maintain a WHC of approximately 50% throughout the experiment. In total, eight experimental groups (three replicates per group) were set up: (i) Original soils (Clean); (ii) Hg-polluted soils with no additive (Control); (iii) Hg-polluted soils with DC-B1 (+DC-B1); (iv) Hg-polluted soils with DC-B2 (+DC-B2); (v) Hg-polluted soils amended with 1% biochar (+1% biochar); (vi) Hg-polluted soils with 4% biochar (+4% biochar); (vii) Hg-polluted soils amended with DC-B1 and 4% biochar (+DC-B1 + 4% biochar); and (viii) Hgpolluted soils with DC-B2 and 4% biochar (+DC-B2 + 4% biochar). After seven days of pretreatment, lettuce seeds were well distributed into the soils at a density of eight seeds/pot to determine the germination rate. The lettuce plants were thinned to three seedlings per pot after germination (7 days) to facilitate their growth and further experimental observation. Lettuce is a popular species of vegetable worldwide and can also be employed as an effective bio-indicator of Hg contamination in soil (Mahbub et al., 2017b). Then, soil and plant samples were harvested for analysis after 56 days of cultivation. A portion of soil samples were airdried and grounded, and the others were freeze-dried and stored at À20°C for microbiological analysis.
Sample analysis. Lettuce plants were separated into above-ground (shoot) and below-ground (root) parts after cleaning with sterile water, and their growth parameters, including lengths of shoots and roots, fresh weight and contents of protein and chlorophyll in shoots, were measured. The protein content was determined using a commercial protein kit (Jiancheng Bioengineering Institute, China), and chlorophyll was extracted using 80% (w/w) acetone and measured by spectrophotometry . Then, the plant was oven-dried at 50°C to a constant weight. Soil and plant samples were digested following the methods described by Zheng et al. (2008) and Han et al. (2006) respectively. The Hg concentration in the solutions was determined using a hydride generation atomic fluorescence spectrometer (AFS-8220; Jitian Analytical Instrument Co., Beijing, China) (Chen et al., 2018). Certified reference soil (GBW-07405, 0.29 AE 0.03 mg Hg kg À1 ) and tomato leaves (CRM 1753a, 34 ng Hg g À1 ) were also determined following the same procedures, and the recovery percentages of Hg were 90-98%.
Responses of the soil bacterial community DNA extraction. Extraction of soil DNA was performed using a PowerSoil â DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer's protocols. The extracted DNA was purified utilizing the TIANquick Midi Purification Kit (TIANGEN, Beijing, China), and its purity was checked by electrophoresis. Then, the product was stored at À20°C until further analysis. Triplicate purified DNA samples from each group were mixed and sequenced as one sample.
Bacterial community analysis. The V3-V4 region of the bacterial 16S rRNA gene was amplified with universal primers 338F (5 0 -ACTCCTACGGGAGGCAGCA-3 0 ) and 806R (5 0 -GGACTACHVGGGTWTCTAAT-3 0 ) . The purified PCR products were analysed using commercial Illumina MiSeq sequencing technology (Person Biotechnology Co., Ltd, Shanghai, China) to obtain bacterial community information following the methods and program described in previous reports Xu et al., 2016). The obtained raw reads were processed using the QIIME Pipeline (Version 1.8.0, http://qiime.org/). After filtering low-quality or ambiguous reads and removing chimeric sequences, the high-quality sequences were clustered into operational taxonomic units (OTUs) at an identity level of 97%, and bacterial taxonomy was assigned using the Ribosomal Database Project (RDP) classifier. Shannon and Simpson indices were calculated to indicate bacterial diversity, and the abundance-based coverage estimator (ACE) and the estimated asymptotic microbial taxon richness (Chao1) were used to evaluate the bacterial richness. The Bray-Curtis distance was calculated using the dominant genera, and a heat map depicting the changes in bacterial communities was drawn using R software.

Statistical analysis
All of the assays in this study were performed in triplicate, and the results were expressed as mean value AE SD. Treatment effects on Hg contents in soils and plants were analysed by one-way analyses of variance (ANOVA) followed by the least significant difference (LSD) using SPSS 23.0, and a difference was considered significant at the P < 0.05 level. All data were tested for normality and homogeneity of variance before ANOVA. Of these data, lettuce shoot length and total biomass were not normally distributed, and Dunnett's T3 post hoc test was applied for analysis.